From 98fac3d2d0a1a518134aa5aab9a374ae28bdfc29 Mon Sep 17 00:00:00 2001 From: Camron Blackburn <camron.blackburn@cba.mit.edu> Date: Mon, 30 Mar 2020 17:19:58 -0400 Subject: [PATCH] Update README.md --- README.md | 19 +++++++++++++++++++ 1 file changed, 19 insertions(+) diff --git a/README.md b/README.md index d0c92f1..4aa2f9a 100644 --- a/README.md +++ b/README.md @@ -68,6 +68,25 @@ Ah - and add in a flowmeter after the second OPC sampler! - andersen sampler?? could be nice to bin different size particles - six-stage viable particle cascade impactor - OPC would be better than andersen sampler + +# particle counting +### optical particle counter + +### fluorescent particle counter +fluorescently labeled microscpheres - https://www.thermofisher.com/order/catalog/product/93470350011150?SID=srch-srp-93470350011150#/93470350011150?SID=srch-srp-93470350011150 \ +Measure particles: \ +CBA has a plate reader - can return flourescent concentration (not individual particle #) but requries optimization \ +or confocal microscope - collect particels in iminger with water solution, let colloidal solution evaporate leaving particles on stage and record particle desnity in an image + + +### microgram mass comparison of gold particles +test measurement with 100nm gold particles \ +from bbi solutions dataseet for 100nm gold particles - mass of 1L of gold is 5.66E-05 g, too small for the analytical balance in BL1 \ +also, I tried measuring evaporated solution on a few microscope slides and the slides are not uniform enough to do comparative measurements. \ +but can see the particles in the Olympus microscope + + + ### Resources [^DIYmask]: University of Cambridge [DIY mask efficacy testing](https://www.researchgate.net/publication/258525804_Testing_the_Efficacy_of_Homemade_Masks_Would_They_Protect_in_an_Influenza_Pandemic) [^filterPerformance]: AR Wilkes [The bacterial and viral filtration performance of breathing system filters](https://onlinelibrary.wiley.com/doi/full/10.1046/j.1365-2044.2000.01327.x) -- GitLab