Commit 2786612a authored by James Pelletier's avatar James Pelletier

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<meta name="generator" content="GitLab Pages"> <meta name="generator" content="GitLab Pages">
<title>Plain HTML site using GitLab Pages!!</title> <title>James Pelletier</title>
<link rel="stylesheet" href="style.css"> <link rel="stylesheet" href="style.css">
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<div class="navbar">
<a href="">Plain HTML Example</a>
<a href="">Repository</a>
<a href="">Other Examples</a>
<h1>Hello World!</h1>
<p> <p>
This is a simple plain-HTML website on GitLab Pages, without any fancy static site generator. Greetings! I am a 6th year graduate student in the Center for Bits and Atoms and the Department of Physics at MIT. I am interested in how nonliving molecules work together to make living systems capable of metabolism, growth, replication, homeostasis, and adaptation. In particular, we are interested in "top-down" and "bottom-up" approaches to synthetic cells. We hope to combine the most complex nonliving molecules, such as cytoplasm and chromosomes, to make the least complex living systems.
From the "top-down" we are investigating a <a href="">bacterium with a minimal genome</a>, in collaboration with John Glass of the <a href="">J. Craig Venter Institute</a> and Elizabeth Strychalski of the <a href="">National Institute of Standards and Technology</a>. For context, humans contain about 20000 genes, the bacterium <i>Escherichia coli</i> contains about 4000 genes, and the minimal cell contains just 473 genes. To our surprise, 149 of them do not have a known function, even though all are essential and the cell would die without any one of them. Therefore, we now know which genes a minimal cell contains, but we do not understand how the genes work. By imaging the growth and replication of single cells in microfluidic devices, we are now investigating <i>how</i> the minimal cell lives.
From the "bottom-up" we are reconstituting cellular phenomena in eukaryotic cytoplasmic extract from frog eggs, with <a href="">Timothy Mitchison</a> and <a href="">Nikta Fakhri</a>. For example, we are investigating geometrical and mechanical aspects of intracellular scaffolds such as microtubules, actin, and endoplasmic reticulum. I enjoy thinking about these cytoskeletal scaffolds as “LEGO soup,” composed of many different molecules that are constantly using energy to self-organize, assembling and disassembling to make different structures that can do different jobs at different times.
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